Travis Murphy, MD
University of Florida
"Adaptation of Piglet Model to Adult Swine Ventricular Fibrillation Arrest Resuscitation with Perfluorocarbon Emulsion"
SAEMF/RAMS Resident Research Grant
The main goal of this investigation is to perform a feasibility study regarding the use of an adapted piglet ventricular fibrillation model of cardiopulmonary resuscitation in adult swine. If successful, we plan to test the effects of perfluorocarbon (PFC) emulsions on post-arrest outcome. PFC emulsions have been shown to reduce the negative effects of anoxia and ischemia but the potential benefit during cardiac arrest is currently unknown. Our research team seeks funding to test this hypothesis in a model that has been approved by our institutional IACUC.
Our central hypothesis is that an adaptation of a previously published piglet model for ventricular fibrillation arrest and resuscitation at this institution will serve as an appropriate model for the trial of PFC use in the resuscitation of adult swine during induced ventricular fibrillation arrest.
The proposed study will be performed with the assistance of veterinarians experienced in swine models. For this initial study, animals will be obtained and baseline cognitive scoring and blood values to reflect oxygenation will be collected. Ventricular fibrillation will be induced via central venous catheter wire placed in the internal jugular vein in anesthetized and mechanically ventilated animals. Standard advanced cardiac life support will be performed after a no-flow period to best represent out-of-hospital cardiac arrest. The expected outcome is the predictable resuscitation of swine models, tolerance of test solution of PFC and reliable evaluation of cognitive function and findings on light microscopy.
If return of spontaneous resuscitation is achieved, blood samples for oxygenation will be drawn and the animals will be observed for neurologic outcomes using previously validated metrics. This will serve as demonstrated feasibility to apply for long-term grant funding to more fully assess the effects of PFC emulsions on oxygenation, microcirculation and neurologic outcome following cardiac arrest.
This project is relevant to the practice of emergency medicine as cardiac arrest and the mitigation of effects on neurologic outcome are central to the care of critically ill patients. We anticipate the findings of this study to demonstrate the feasibility of this model as a vehicle for assessing the effect of PFC emulsions on neurologic outcomes after cardiac arrest.
Dr. Murphy is still completing the project.